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生物化学与分子生物学
日期:2018-01-24 信息来源: 点击数:

生物化学与分子生物学学科简介

学科带头人:盛清
学科组主任:聂作明
学科组副主任:于威、潘建义

生物化学与分子生物学学科依托浙江省生物学重中之重一级学科和浙江省家蚕生物反应器与生物医药重点实验室,具有一支结构合理的科研教学队伍,共有教师20人,其中教授4人,副教授(副研究员)7人,讲师9人,博士14人,硕士研究生导师9,在读研究生近40。学科实验室设备条件完善,可开展功能基因组学和蛋白质组学研究以及分子生物学、微生物学、免疫学、组织化学、免疫化学、病理学、组织学毒理学等实验。近3年来先后承担了包括国家重点基础研究发展计划(973计划)、国家高技术研究发展计划(863计划)和国家自然科学基金等国家级项目12项,其它各类省部级项目7项,总经费1500余万元;近3年来发表论文80余篇,其中SCI论文35,申请专利23项,其中授权专利7。本学科经过几年的发展,形成了肿瘤生化与分子生物学、病毒生化与分子生物学、疾病相关蛋白质组学、基因表达与调控和非编码RNA的鉴定与功能5个特色研究方向在肿瘤耐药分子机制杆状病毒和乙肝病毒侵染机制防治、重大疾病关键蛋白的鉴定、家蚕功能基因组学和以家蚕为模式生物探索鳞翅目害虫生物防治新方法方面取得了较好的成果。生物化学与分子生物学学科在学校和学院的领导下,艰苦奋斗、求真务实、勇于创新,正走上一条蓬勃发展的道路。

近三年省部级以上项目

1. 家蚕生物反应器产业化体系与产品开发,国家863重点项目,2011.6-2015.12836万元

2. 家蚕关键品质性状分子解析及分子育种基础研究,国家973项目子课题,2012.1-2016.1280万元

3. 耐辐射奇球菌抗辐射相关非编码小RNA及其靶标mRNA的鉴定,国家自然科学基金,2012.1-2014.1221万元

4. 猪圆环病毒2型感染中NF-κB信号通路的激活机制及其对细胞凋亡的作用研究,国家自然科学基金,2012.1-2014.1220万元

5. bmo-miR-3378参与家蚕杆状病毒免疫应答调控机制的研究,国家自然科学基金,2012.1-2015.1220万元

6. mir-27a负调控I型干扰素的分子机制研究,国家自然科学基金,2014.1-2016.1223万元

7. 铁离子调控大肠埃希菌O157:H7外膜孔蛋白OmpW 抗先天免疫的分子机制,国家自然科学基金,2013.1-2015.1223万元

8. 哺乳动物速激肽hemokinin-1的中枢镇痛位点和镇痛机制研究,国家自然科学基金,2011.1-2013.1221万元

9. 纳米管形大碳笼金属富勒烯的合成、结构及其在光伏电池中的应用,国家自然科学基金,2013.1-2016.1289万元

10. 基于iTRAQ技术高通量筛选梅毒血清固定血清学诊断标志物,国家自然科学基金,2014.01-2017.1240万元

11. 靶向阻断神经激肽受体抑制白血病细胞生长的分子机制及其在白血病治疗中的潜在价值,国家自然科学基金,2015.1-2016.1230万元

12. 金属富勒烯的质谱研究国家自然科学基金,2015.1-2017.1225万元

13. 家蚕bmo-miR-7miR-79靶基因的筛选及调控Notch途径靶基因的鉴定,浙江省自然科学基金,2012.1-2013.128万元

14. 家蚕杆状病毒bm64基因的功能研究,浙江省自然科学基金,2011.1-2013.125万元

15. 铜绿假单胞菌PpkA磷酸化网络及其对耐药的调控机制研究,浙江省自然科学基金,2015.1-2017.1210万元

16. 沙瑞度坦抑制人粒性白血病细胞增殖的靶点蛋白筛选及其功能研究,浙江省自然科学基金,2014.01-2016.128万元

17. 金属富勒烯复杂体系的质谱分析研究浙江省自然科学基金,2014.1-2016.125万元

18. 家蚕贮藏蛋白SP乙酰化修饰鉴定及其上调蛋白表达的分子机制,浙江省自然科学基金,2016.1-2018.1210万元

19. PIMTHsp27相互作用在DOX诱导乳腺癌细胞凋亡中的机制研究,浙江省自然科学基金,2016.1-2018.125万元

近三年主要横向项目

1. miR-27a调控RNA病毒感染诱导的I 型干扰素的产生及其分子机制的研究,2014.9-2015.124万元

2. 栗子坪自然保护区大熊猫主食竹区域土壤酶活性测定,2013.12-2015.12

3. 猪圆环病毒II型抗体检测试剂盒的开发,2015-1-2016.62万元

4. 蜂毒抗菌肽Anoplin在食管癌治疗中的用途2015.01-2024.1

5. 肺癌小分子靶向药物的体外药效学及药代动力学研究2013.04-2014.1215万元

6. 重组大流行流感灭活疫苗(家蚕杆状病毒)相关毒种、细胞的研究2013.01-2014.1210万元

7. 神经激肽受体拮抗剂在制备抗癌药物中的用途,2015.01-2024.1

8. 复合型水体修复剂研制2013.9-2015.432.5万元

9. 蛋白质翻译后修饰鉴定的组学技术研发,2014.01-2015.1210万元

近三年发表主要论文

(1) Ou WB, Zhu J, Eilers G, Li X, Kuang Y, Liu L, Mariño-Enríquez A, Yan Z, Li H, Meng F, Zhou H, Sheng Q, Fletcher JA. HDACi inhibits liposarcoma via targeting of the MDM2-p53 signaling axis and PTEN, irrespective of p53 mutational status. Oncotarget, 2015,6(12):10510-20.

(2) Nie Z, Zhu H, Zhou Y, Wu C, Liu Y, Sheng Q, Lv Z, Zhang W, Yu W, Jiang C, Xie L, Zhang Y, Yao J. Comprehensive profiling of lysine acetylation suggests the widespread function is regulated by protein acetylation in the silkworm, Bombyx mori. Proteomics, 2015,15(18):3253-66.

(3) Wu C, Wang C, Li D, Liu Y, Sheng Q, Lv Z, Yu W, Nie Z. BmHSP20.8 is Localized in the Mitochondria and has a Molecular Chaperone Function In Vitro. J Insect Sci. 2015,15(1):99.

(4) Shi Y, Zhang C, Xie C, Quan Y, Nie Z, Chen J, Lv Z, Zhang Y, Yu W. The effect of Bm67 gene deletion on Bombyx mori nuclear polyhedrosis virus replication. Acta Virol. 2015, 59(1):40-8.

(5) Pan J, Chen R, Li C, Li W, Ye Z. Global Analysis of Protein Lysine Succinylation Profiles and Their Overlap with Lysine Acetylation in the Marine Bacterium Vibrio parahemolyticus. J Proteome Res. 2015, 14(10):4309-18.

(6) Wang KZ, Weng LY, Ye ZC, Wu HG, Pan JY. Hemocyanin-derived phenoloxidase activity is dependent on dodecameric structure in shrimp Litopenaeus vannamei. Arch Biol Sci. 2015, 67(1):19−23.

(7) Yu M, Qiu Y, Chen W, Zhao F, Shao J. Action modes of recombinant endocellulase, EGA, and its domains on cotton fabrics. Biotechnol Lett, 2015, 37(8):1615-22.

(8) Yu M, Huang M, Song Q, Shao J, Ying X. Characterization of a (2R,3R)-2,3-Butanediol Dehydrogenase from Rhodococcus erythropolis WZ010. Molecules, 2015, 20(4):7156-73.

(9) Zhu XC, Zhang JL, Ge CT, Yu YY, Wang P, Yuan TF, Fu CY. Advances in cancer pain from bone metastasis. Drug Design, Development and Terapy, 2015, 9: 4239-4245.

(10) Zhu XC, Ge CT, Wang P, Zhang JL, Yu YY, Fu CY. Analgesic effects of lappaconitine in leukemia bone pain in a mouse model. Peer J, 2015, 3:e936. DOI 10.7717/peerj.936.

(11) Xuan Li, Zhi-Rong L, Dong Shen, Yi Zhan, Jun-Mo Yang, Yong-Doo Park, Hai-Meng Zhou, Qing Sheng, Jinhyuk Lee. The inhibitory role of Co2+on _-glucosidase: Inhibition kinetics andmolecular dynamics simulation integration study. Process Biochemistry, 2014,49:1913-1919.

(12) Liu M, Wang C, Li D, Liu Y, Sheng Q, Lv Z, Yu W, Wang D, Zhang Y, Nie Z. Cloning and expression characteristics of the notch-associated gene BmE(spl)mγ from silkworm, Bombyx mori. Appl Biochem Biotechnol, 2014, 173(8):2065-75.

(13) Zheng Q, Sheng Q, Jiang C, Shu J, Chen J, Nie Z, Lv Z, Zhang Y. MicroRNA-452 promotes tumorigenesis in hepatocellular carcinoma by targeting cyclin-dependent kinase inhibitor 1B. Mol Cell Biochem, 2014, 389(1-2):187-95.

(14) Pan J, Ye Z, Cheng Z, Peng X, Wen L, Zhao F. Systematic Analysis of the Lysine Acetylome in Vibrio parahemolyticus. J Proteome Res, 2014, 13(7):3294−3302

(15) Li C, Ye Z, Wen L, Chen R, Tian L, Zhao F, Pan J. Identification of a novel vaccine candidate by immunogenic screening of Vibrio parahaemolyticus outer membrane proteins. Vaccine, 2014, 32(46): 6115–6121.

(16) Fu CY, Xia RL, Zhang TF, Lu Y, Zhang SF, Yu ZQ, Jin T, Mou XZ. Hemokinin-1(4-11)-induced analgesia selectively up-regulates δ-opioid receptor expression in mice. PLoS ONE, 2014, 9(2): e90446.

(17) Fu CY, Chen W, Jin YT. The complete mitochondrial genome of Phrynocephalus guinanensis (Reptilia, Squamata, Agamidae). Mitochondrial DNA, 2014, 1-2.

(18) M L Mo, M R. Li, Z. Chen, X W. Liu, Q Sheng*, H.M. Zhou*. Inhibition of the Wnt palmitoyltransferase porcupine suppresses cell growth and downregulates the Wnt/β-catenin pathway in gastric cancer. Oncol Lett, 2013, 5(5):1719-1723.

(19) Nie Z, Zhou F, Li D, Lv Z, Chen J, Liu Y, Shu J, Sheng Q, Yu W, Zhang W, Jiang C, Yao Y, Yao J, Jin Y, Zhang Y. RIP-seq of BmAgo2-associated small RNAs reveal various types of small non-coding RNAs in the silkworm, Bombyx mori. BMC Genomics, 2013, 14:661.

(20) Yu W, Ying H, Tong F, Zhang C, Quan Y, Zhang Y. Protective effect of the silkworm protein 30Kc6 on human vascular endothelial cells damaged by oxidized low density lipoprotein (Ox-LDL). PLoS One, 2013, 8(6):e68746.

(21) Zhou F, Gao Z, Lv Z, Chen J, Hong Y, Yu W, Wang D, Jiang C, Wu X, Zhang Y, Nie Z*. Construction of the ie1-Bacmid Expression System and Its Use to Express EGFP and BmAGO2 in BmN Cells. Appl Biochem Biotechnol, 2013, 169(8):2237-2247.

(22) Yu W, Du C, Quan Y, Nie Z, Chen J, Lv Z, Zhang Y. Characterization of late gene expression factor LEF-10 from Bombyx mori nucleopolyhedrovirus. Virus Research, 2013,175: 45-51.

(23) Yu W, Li Q, Yao Y, Quan Y, Zhang Y. Two novel 30K proteins overexpressed in baculovirus system and their anti-apoptotic effect in insect and mammalian cells. International Journal of Genomics, 2013, 323592.

(24) Wu XB, Tian LH, Zou HJ, Wang CY, Tang CH, Yu ZQ, Zhao FK, Pan JY. Outer membrane protein OmpW of Escherichia coli is required for resistance to phagocytosis. Res Microbiol. 2013, 164(8): 848-855.

(25) Pan JY, Wu H, Liu X, Li PP, Li H, Wang SY, Peng XX. Complexome of Escherichia coli cytosolic proteins under normal native conditions. Mol Biosyst. 2011, 7(9):2651-2663.

(26) RL, Lu Y, Zhu LN, Zhang SF, Zhao FK, Fu CY. Different regulatory pathways are involved in the proliferative inhibition of two types of leukemia cell lines induced by paclitaxel. Oncology Reports, 2013, 30: 1853-1859.

(27) Zhu LN, CY, SF, Chen W, YT, Zhao FK. Novel cytotoxic exhibition mode of antimicrobial peptide anoplin in MEL cells, the cell line of murine Friend leukemia virus- induced leukemic cells. Journal of Peptide Science, 2013, 19: 566-574.

(28) Xia RL, Fu CY, Zhang SF, Jin YT, Zhao FK. Study on the distribution sites and the molecular mechanism of analgesia after intracerebroventricular injection of rat/mouse hemokinin-1 in mice. Peptides, 2013, 43, 113-120.

近三年授权专利

1.一种以大肠杆菌外膜孔蛋白ompW为靶点筛选药物的方法;专利授权号:201210275708.9

2.神经激肽受体拮抗剂在制备抗癌药物中的用途;专利授权号:ZL 20111 0070470.1

3. SR140333的抗血癌作用;专利授权号:ZL 2011 1 0070497.0

4.神经激肽受体2的拮抗剂在白血病治疗中的用途;专利授权号:ZL 2012 1 0323180.8

5.蜂毒抗菌肽Anoplin在食管癌治疗中的用途;专利授权号:ZL 2013 1 0069107.7

6.蜂毒抗菌肽Anoplin在肝癌治疗中的用途;专利授权号:ZL 2013 1 0068642.0

7.蜂毒抗菌肽Anoplin在血癌治疗中的用途;专利授权号:ZL 2013 1 0069106.2

(2015.11.17)





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